Mye-EUNITER
 

Baekkevold, Espen

Models available:

Human blood and tissue macrophages and DCs, airway mucosa, intestinal mucosa, functional and molecular characterization of sorted subsets, turnover kinetics in organ transplanted patients, experimentally induced allergic rhintis, in vivo gluten challenge celiac disease, chemokines and recruitment of macrophage and DC precursors.

In our projects, we utilize novel strategies to study the heterogeneity and functional specializations of MRCs like macrophages and DCs in human tissues, with focus on the intestinal and airway mucosa during homeostasis and inflammatory disease (allergy and celiac disease). 

Cells available:

Monocytes, DCs, macrophages.

Technology available:

14-color flow cytometry, and 8 color FACS-sorting. We have developed techniques for tissue dispersion and flow-cytometric phenotyping and sorting of tissue cells, including macrophages and DCs. Together with our clinical collaborators, we have developed protocols for experimental induction of airway allergy and celiac disease, followed by tissue biopsy and cell isolation and characterization. We have also developed approaches to directly assess the turnover kinetics of human tissue macrophages and DCs in tissues from solid organ transplanted patients.

Key publications related to Mye-EUNITER:

  1. Melum GR, Farkas L, Scheel C, Van Dieren B, Gran E, Liu YJ, Johansen FE, Jahnsen FL, Baekkevold ES. A thymic stromal lymphopoietin-responsive dendritic cell subset mediates allergic responses in the upper airway mucosa. J Allergy Clin Immunol. 2014 Sep;134(3):613-621.

  2. Bjerkan L, Schreurs O, Engen SA, Jahnsen FL, Baekkevold ES, Blix IJ, Schenck K. The short form of TSLP is constitutively translated in human keratinocytes and has characteristics of an antimicrobial peptide. Mucosal Immunol. 2014 May 21.doi: 10.1038/mi.2014.41.

  3. Danilova E, Skrindo I, Gran E, Hales BJ, Smith WA, Jahnsen J, Johansen FE, Jahnsen FL, Baekkevold ES. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa. Mucosal Immunol. 2014 Jun 11. doi: 10.1038/mi.2014.46.

  4. Ráki M, Beitnes AC, Lundin KE, Jahnsen J, Jahnsen FL, Sollid LM. Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion. Mucosal Immunol. 2013 Sep;6(5):985-92. doi:10.1038/mi.2012.136.

  5. Beitnes AC, Ráki M, Brottveit M, Lundin KE, Jahnsen FL, Sollid LM. Rapid accumulation of CD14+CD11c+ dendritic cells in gut mucosa of celiac disease after in vivo gluten challenge. PLoS One. 2012;7(3):e33556. doi:10.1371/journal.pone.0033556.

Contact details:

Oslo University Hospital
Dept. of Pathology
Centre for Immune Regulation (CIR)
0424 Oslo
Norway
Tel. +4723071493
espen.s.bakkevold@rr-research.no