Mye-EUNITER
 

Barczyk-Kahlert, Katarzyna

Cells and models available:

  • human peripheral monocytes.
  • murine monocytes/macrophages.
  • Glucocorticoid-induced anti-inflammatory monocytes (regulatory monocytes, Mregs) of human and mouse origin - characterization and potential role in autoimmune and chronic diseases.
  • CD163-positive macrophages in human and mouse.
  • Role of CD163-positive macrophages in infectious and autoimmune diseases (mouse experimental models).

 

Technology available:

Flow cytometry.
Functional assays.
ELISA and flow cytometry-based measurements of cytokines.
Signal transduction.
Gene expression profiling.
Mouse experimental models of infectious and autoimmune diseases.

 

Key publications related to Mye-EUNITER:

  1. Kneidl J, Mysore V, Geraci J, Tuchscherr L, Loffler B, Holzinger D, Roth J, Barczyk-Kahlert K. Soluble CD163 masks fibronectin-binding protein A-mediated inflammatory activation of Staphylococcus aureus infected monocytes. Cell Microbiol. 2014; 16:364-77.
  2. Kneidl J, Loffler B, Erat MC, Kalinka J, Peters G, Roth J, Barczyk K. Soluble CD163 promotes recognition, phagocytosis and killing of Staphylococcus aureus via binding of specific fibronectin peptides. Cell Microbiol. 2012; 14:914-36.
  3. Tsianakas A, Varga G, Barczyk K, Bode G, Nippe N, Kran N, Roth J, Luger TA, Ehrchen J Sunderkoetter C. Induction of an anti-inflammatory human monocyte subtype is a unique property of glucocorticoids, but can be modified by IL-6 and IL-10. Immunobiology. 2012; 217:329-35.
  4. Barczyk K, Ehrchen J, Tenbrock K, Ahlmann M, Kneidl J, Viemann D, Roth J. Glucocorticoids promote survival of anti-inflammatory macrophages via stimulation of adenosine receptor A3. Blood. 2010; 116:446-55.
  5. Ehrchen J, Steinmuller L, Barczyk K, Tenbrock K, Nacken W, Eisenacher M, Nordhues U, Sorg C, Sunderkotter C, Roth J. Glucocorticoids induce differentiation of a specifically activated, anti-inflammatory subtype of human monocytes. Blood. 2007; 109:1265-74.

 

Contact details:

University of Muenster
Institute of Immunology
Roentgenstr. 21
48149 Muenster
Germany

Telephone: +49 251 83 52945

email: bar@uni-muenster.de