Jonjic, Stipan

Models available:

  • CMV infection/congenital CMV infection
  • Immunoevasion, innate and adaptive immune response in murine (for MCMV) and humanized mice (for HCMV) models. 

Cells available:

  • NK-, T-, B-cells, dendritic cells, macrophages, primary and immortalized embrionic fibroblasts.

Technology available:

  • Humanized NOG-HLA-A2 mice reconstituted with hematopoetic stem cells from the human fetal liver.
  • State of the art SPF animal facility harbouring >100 mouse strains and 12,000 mice.
  • Multicolor flow analysis and cell sorting (FACSAria I and FACSVerse).
  • Custom production/purification and development of proteins/mAbs/hybridomas, including production of biotinylated monoclonal antibodies and precoated ELISA plates, as well as mAb fragmentation (Fab’ & F(ab')2. Extensive mAb database.
  • Cloning and whole transcriptome shotgun sequencing and data analysis (RNASeq).
  • Immunohistology and cellular immunology.

Key publications related to Mye-EUNITER:

  1. Slavuljica I, Busche A, Babić M, Mitrović M, Gašparović I, Cekinović Đ, Markova Car E, Pernjak Pugel E, Ciković A, Juranić Lisnić V, Britt WJ, Koszinowski U, Messerle M, Krmpotić A, Jonjić S. (2010) Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties. J Clin Invest. 120(12):4532-45.

  2. Babić M, Pyzik M, Zafirova B, Mitrović M, Butorac V, Lanier LL, Krmpotić A, Vidal SM, Jonjić S. (2010) Cytomegalovirus immunoevasin reveals the physiological role of „missing self“ recognition in NK-cell dependent virus control in vivo. J Exp Med. 207(12):2663-2673.

  3. Mitrovic M, Arapovic J, Jordan S, Fodil-Cornu N, Ebert S, Vidal SM, Krmpotic A, Reddehase MJ, Jonjic S. (2012) The NK-cell response to mouse cytomegalovirus infection affects the level and kinetics of the early CD8+ T-cell response. J Virol 86(4):2165-75.

  4. Trsan T, Busche A, Abram M, Wensveen FM, Lemmermann NA, Arapovic M, Babic M, Tomic A, Golemac M, Brinkmann MM, Jäger W, Oxenius A, Polic B, Krmpotic A, Messerle M, Jonjic S. (2013) Superior induction and maintenance of protective CD8 T cells in mice infected with MCMV vector expressing RAE-1γ. PNAS, 110(41):16550-5.

  5. Juranic Lisnic V, Babic Cac M, Lisnic B, Trsan T, Mefferd A, Mukhopadhyay CD, Cook CH, Jonjic S, Trgovcich J (2013). Dual Analysis of the Murine Cytomegalovirus and Host Cell Transcriptomes Reveal New Aspects of the Virus-Host Cell Interface. PLoS Pathogens, DOI: 10.1371/journal.ppat.1003611.