Mye-EUNITER
 

Knapp, Sylvia

Models available:

Animal (mouse) models of infection and inflammation:

  • E. coli sepsis, LPS shock, LPS pneumonitis, pneumonia: Strep pneumoniae, Pseudomonas, Acinetobacter; acute lung injury model (acid aspiration), HFD-induced diabetes, influenza.

Cells available:

  • Macrophages

Technology available:

  • Flow Cytometry (14 colors), FACS-sorting, IVIS;
  • Functional macrophage assays: phagocytosis, killing, respiratory burst, inflammatory response, polarization and plasticity.
  • BM-chimera, cell depletion and adoptive transfer studies.
  • Immunohistochemistry
  • Access to NGS and proteomics

Key publications related to Mye-EUNITER:

  1. Sharif, O., R. Gawish, J. M. Warszawska, R. Martins, K. Lakovits, A. Hladik, B. Doninger, J. Brunner, A. Korosec, R. E. Schwarzenbacher, T. Berg, R. Kralovics, J. Colinge, I. Mesteri, S. Gilfillan, A. Salmaggi, A. Verschoor, M. Colonna, and S. Knapp. (2014) The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathogens 10: e1004167.

  2. Warszwaska, J. W., R. Gawish, O. Sharif, S. Sigel, B. Doninger, K. Lakovits, I. Mesteri, M. Nairz, L. Boon, A. Spiel, V. Fuhrmann, B. Strobl, M. Müller, P. Schenk, G. Weiss, and S. Knapp. (2013) Lipocalin-2 deactivates macrophages and worsens pneumococcal pneumonia outcomes. J Clin Invest 123: 3363-3372.

  3. Matt, U., O. Sharif, R. Martins, T. Furtner, L. Langeberg, R. Gawish, I. Elbau, A. Zivkovic, K. Lakovits, O. Oskolkova, B. Doninger, A. Vychytil, T. Perkmann, G. Schabbauer, C. J. Binder, V. N. Bochkov, J. D. Scott, and S. Knapp. (2013) WAVE-1 mediates suppression of phagocytosis by phospholipid-derived DAMPs. J Clin Invest 123: 3014-3024.

  4. Baumann, C. L.*, Aspalter I.M.*, Sharif O*., Pichlmair A, Blüml S, Grebien F, Bruckner M, Pasierbek P, Aumayr K, Planyavsky M, Bennett KL, Colinge J, Knapp S#, Superti-Furga G#. (2010) CD14 is a coreceptor of Toll-like receptors 7 and 9. J Exp Med 207: 2689-2701. * first authors, # corresp. Authors

Contact details:

Research Center for Molecular Medicine of the Austrian Academy of Sciences
Medical University of Vienna
Dept. of Medicine 1, Laboratory of Infection Biology
Waehringer Guertel 18-20
1090 Vienna, Austria
Phone +43-1/40400-51390Fax +43-1/40400-51670
sylvia.knapp@meduniwien.ac.at