Mye-EUNITER
 

Winter, Nathalie

Models available:

  • Tuberculosis and other mycobacterial infections.
  • Mainly in mouse. Different mouse lines.
  • Possible to run pig and cattle infections in BSL3 contained facilities

Cells available:

  • Neutrophils
  • Myeloid-derived-suppressor cells

Technology available:

  • Flow cytometry (recent acquisition of four lasers equipment for 8 to 12 colors)
  • Confocal microscopy, IVIS imaging, 
  • Cellular immunology
  • Immunohistochemistry, Laser microdissection
  • Bacterial assays (in BSL2 and 3 facilities)

Key publications related to Mye-EUNITER:

  1. E. Doz, R. Lombard, F. Carreras, D. Buzoni-Gatel and N. Winter (2013) Mycobacteria-infected dendritic cells attract neutrophils that produce IL-10 and specifically shut down Th17 CD4 T cells through their IL-10 receptor. . J. Immunol, 191, 3818-3826.

  2. A. Martino, E. Badell, V. Abadie, V. Balloy, M. Chignard, M.Y. Mistou, B. Combadière, C. Combadièreet N . Winter (2010) : Mycobacterium bovis BCG vaccination mobilizes innate myeloid-derived suppressor cells restraining in vivo T cell priming via IL-1R-dependent NO production. J. Immunol., 184, 2038–2047

     

  3. D. Bréa ; F. Meurens ; A.V. Dubois ; J. Gaillard ; C. Chevaleyre ; M.L. Jourdan, N. Winter ; B. Arbeille, M. Si-Tahar ; F. Gauthier et S. Atucci (2012) : The pig as a model for investigating the role of neutrophil serine protéases in human inflammatory lung diseases. Biochem. J,447, 363-370

  4. C. Morel, E. Badell V. Abadie,M. Robledo, N. Setterblad, J. C. Gluckman, B. Gicquel, S.BoudalyandN. Winter (2008) Mycobacterium bovis BCG-infected neutrophils and dendritic cells cooperate to induce specific T-cell responses in humans and mice. Eur. J. Immunol., 38, 437-447

  5. V. Abadie, E. Badell, P. Douillard, D. Ensergueix, P.J. Leenen, M. Tanguy, L. Fiette, S. Saeland, B. Gicquel and N. Winter (2005) : Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph node. Blood, 106, 1843-1850

Contact details:

Institut National de la Recherche Agronomique (INRA)
Centre Val de Loire
Infectiologie et Sante Publique
37380 Nouzilly
France
Phone +33 2 47 42 73 14
Fax +33 3 47 42 72 79
nathalie.winter@tours.inra.fr